ELISA PathRF: Human TNF‑α

ELISA PathRF: Human TNF‑α

3512-1HP-DP-1

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Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

PlatePre-coated ELISA strip plate (mAb MT25C5)
Detection mAbMTR-9, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate
StandardRecombinant human TNF-α ELISA standard
SubstrateTMB substrate for ELISA
Buffers/SolutionsRF-block diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human TNF-α in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

ProductELISA PathRF: Human TNF-α
ApplicationELISA
AnalyteTNF-α
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity2 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human TNF-α.
Datasheet/Protocol
SDS

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Featured product, Published March 1, 2022

How to mitigate interference caused by rheumatoid factor
Analyzing samples from patients with ELISA? Watch out for heterophilic antibodies causing false positives. Discover how Mabtech’s kits overcome this issue.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124