ELISA Pro: Human IL‑1β

3416-1HP-1

Publications: 39

Documents

Bulk or custom format

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb MT175)
Detection mAb	7P10, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Recombinant human IL-1β ELISA standard
Substrate	ELISA substrate: TMB for HRP
Buffers/Solutions	ELISA diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IL-1β in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human IL-1β
Application	ELISA
Analyte	IL-1β
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).
Cross-reactivity	The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	1-316 pg/ml
Sensitivity	0.4 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.

Representative standard curve for human IL-1β ELISA Flex kit using TMB substrate.

Documents

Datasheet/Protocol
SDS

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Publications (39)

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Analyte information

IL-1β

Analyte description	Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
Alternative names	Interleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell type	Monocyte/MΦ, mDC
Gene ID	3553