ELISA Pro: Mouse IL‑12 p70

ELISA Pro: Mouse IL‑12 p70

3456-1HP-1

Publications: 21
Documents
Bulk or custom format
Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

PlatePre-coated ELISA strip plate (mAb C17.8)
Detection mAbIL12-IV, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IL-12 p70 ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse IL-12 p70 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Mouse IL-12 p70
ApplicationELISA
AnalyteIL-12 (p70)
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-12 p70.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range30-10000 pg/ml
Sensitivity6 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 4 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of mouse IL-12 (p70) ELISA Pro kit.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-12 (p70)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Alternative namesInterleukin 12, IL-12, IL12, IL-12p35, Il-12a, Ll12a, p35, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16159, 16160