ELISA Pro: Mouse IL-12 p70

ELISA Pro: Mouse IL‑12 p70

3456-1HP-1

Publications: 21
Documents
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

PlatePre-coated ELISA strip plate (mAb C17.8)
Detection mAbIL12-IV, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IL-12 p70 ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse IL-12 p70 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Mouse IL-12 p70
ApplicationELISA
AnalyteIL-12 (p70)
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-12 p70.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range30-10000 pg/ml
Sensitivity6 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 4 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of mouse IL-12 (p70) ELISA Pro kit.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published July 31, 2023

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Tutorial, Published November 28, 2022

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IL-12 (p70)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Alternative namesInterleukin 12, IL-12, IL12, IL-12p35, Il-12a, Ll12a, p35, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16159, 16160