ELISA Pro: Mouse TNF-α

ELISA Pro: Mouse TNF‑α

3511-1HP-1

Publications: 35
Documents
Questions?
Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

PlatePre-coated ELISA strip plate (mAbs MT1C8/23C9)
Detection mAbMT11B10, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse TNF-α ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of mouse TNF-α in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Pro: Mouse TNF-α
ApplicationELISA
AnalyteTNF-α
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range3.16-1000 pg/ml
Sensitivity2 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of mouse TNF-α ELISA Pro kit.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID21926