ELISA substrate: pNPP for ALP

3652-P10

Special offer

You may add these complementary products at a reduced price.

ELISA conjugate: Streptavidin‑ALP

Reactivity:
Application:
Plates:

This offer is valid when purchasing ELISA substrate: pNPP for ALP or other qualifying products.

Publications: 3

Documents

Questions?

Validated for our assays.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 1

ELISA conjugate: Streptavidin-ALP

Product specifications

Intended use

This ELISA substrate is intended for the development of ELISA assays using Strepdavidin-ALP enzyme-conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The high sensitivity pNPP ELISA substrate is supplied in diethanolamine buffer as a ready-to-use solution. It is recommended for both endpoint and kinetic determinations. The substrate produces a soluble yellow product which is measured at 405-410 nm.

We recommend this substrate for our ELISA Flex kits (ALP) and Streptavidin-ALP.

Recommendation

Add the substrate after incubation with alkaline phosphatase and subsequent washing. Incubate for typically 30-90 minutes at room temperature. The substrate produces a yellow product. Read the absorbance at 405-410 nm.

Product details

Product	ELISA substrate: pNPP for ALP
Application	ELISA
Contents	Ready-to-use pNPP substrate for ELISA

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 12 months from date of receipt.

Documents

Datasheet/Protocol SDS

Publications (3)

Loading publications...