ELISpot Flex: Bovine IL‑8 (HRP)

ELISpot Flex: Bovine IL‑8 (HRP)

3114-2H

Publications: 1
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT8H6/8F19
Detection mAb26E5, biotin
Enzyme conjugateStreptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting bovine IL-8 (CXCL8) using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Bovine IL-8 (HRP)
ApplicationELISpot
AnalyteIL-8 (CXCL8)
ReactivityCow, Dog
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of bovine (cow) IL-8 (CXCL8).
Cross-reactivityThe mAbs cross-react with IL-8 from dog. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/ProtocolSDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IL-8 (CXCL8)

Analyte description

Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.

Alternative namesInterleukin-8, IL-8, IL8, CXCL8
Cell typeT cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID280828, 403850
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ELISpot Plus: Bovine IL-8 (HRP)