ELISpot Flex: Mouse IgG2a (HRP)

3822-2H

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Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT24/JC5-1
Detection mAb	MG2a, biotin
Enzyme conjugate	Streptavidin-HRP
Stimuli	R848
	Recombinant mouse IL-2

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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Anti-mouse IgG2a mAb (MG2a), unconjugated

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2a using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2a secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

The IgG2a allele is expressed in e.g., Balb/c mice.

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

Product	ELISpot Flex: Mouse IgG2a (HRP)
Application	ELISpot
Analyte	IgG2a
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG2a.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Mouse IgG2a ELISpot using in vivo activated spleen cells from Balb/c mice (100.000 cells/well).

Documents

Datasheet/Protocol SDS

Mouse IgG2a mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2a. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2a antibody-secreting cells.

Application guide: ELISpot

Ag-specific IgG2a

Coating:
Antigen
Detection mAb:
MG2a, biotin

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
Antigen, biotin

Total IgG2a

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
MG2a, biotin

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (?)

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Analyte information

IgG2a

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2a allele is expressed in e.g., Balb/c mice.
Alternative names	Immunoglobulin G2a, IgG2a, 1810060O09Rik, Igh-1, Igh-1a
Cell type	B cell
Gene ID	380793