ELISpot Flex: Mouse IgG2c (ALP)

ELISpot Flex: Mouse IgG2c (ALP)

3824-2A

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT24/JC5-1
Detection mAbMTG2c, biotin
Enzyme conjugateStreptavidin-ALP
StimuliR848
Recombinant mouse IL-2

In stock

Delivery 4-9 business days

Shipping $0

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Anti-mouse IgG2c mAb (MTG2c), unconjugated


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2c using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2c secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

The IgG2c allele is expressed in certain inbred mouse strains e.g., C57BL/6, C57BL/10, SJL and NOD. 

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

ProductELISpot Flex: Mouse IgG2c (ALP)
ApplicationELISpot
AnalyteIgG2c
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG2c.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Splenocytes from C57/BL6 mice were pre-incubated for 72 h in the absence or presence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells and analyzed for the secretion of IgG2c (50,000 cells/well, overnight incubation).
Datasheet/ProtocolSDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2c. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2c antibody-secreting cells.

 

Application guide: ELISpot

 

Antigen-specific IgG2c

Coating:
Antigen
Detection mAb:
MTG2c, biotin

mouse igg2c 1

 

Coating mAbs:
MT24/JC5-1, unconjugated
Detection:
Antigen

mouse igg2c 2

Total IgG2c

Coating mAbs:
MT24/JC5-1, unconjugated
Detection mAb:
MTG2c, biotin

mouse igg2c 3

 

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IgG2c

Analyte description

IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins.  In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.

Alternative namesImmunoglobulin G2c, IgG2c
Cell typeB cell