ELISpot Flex: Mouse IL‑1α (HRP)

3417-2H

Publications: 0

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT946
Detection mAb	MT951, biotin
Enzyme conjugate	Streptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: TMB for HRP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-1α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Mouse IL-1α (HRP)
Application	ELISpot
Analyte	IL-1α
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IL-1α.
Cross-reactivity	The mAbs do not cross-react with mouse IL-1β.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

Mouse spleen cells were incubated for 20 hours in an ELISpot plate. Cells were unstimulated or stimulated with LPS (200,000 cells/well). The number of cells secreting IL-1α was analyzed by ELISpot.

Documents

Datasheet/Protocol SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (?)

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Analyte information

IL-1α

Analyte description	Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation.
Alternative names	Interleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, Il-1a
Cell type	Monocyte/MΦ, mDC
Gene ID	16175