ELISpot Flex: Rat IL‑22 (HRP)

ELISpot Flex: Rat IL‑22 (HRP)

3275-2H

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT230
Detection mAbMT231, biotin
Enzyme conjugateStreptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting rat IL-22 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Rat IL-22 (HRP)
ApplicationELISpot
AnalyteIL-22
ReactivityRat
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of rat IL-22.
Cross-reactivityThe mAbs react with mouse IL-22 and cross-react with IL-22 from rat. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Rat spleen cells were incubated overnight in the absence or presence of PMA/ionomycin and the number of cells secreting IL-22 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

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Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-22

Analyte description

The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.

Alternative namesInterleukin-22, IL-22, IL22, IL-22a, ILTIFa, If2b1, Iltif
Cell typeT cell, Th1, Th17, Tfh
Gene ID50929