ELISpot Plus: Bovine IL‑2 (ALP)

ELISpot Plus: Bovine IL‑2 (ALP)

3111-4APW-2

Publications: 0
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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT11A31)
Detection mAbMT3B3, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Delivery within 3 weeks

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC from cow were incubated in the presence or absence of Phytohaemagglutinin (PHA) and the number of cells secreting IL-2 was analyzed by bovine IL-2 ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 7, 2022

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Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID280822