ELISpot Plus: Bovine IL‑2 (ALP)

Bovine Interleukin-2 (IL-2) ELISpot. PBMC from cow incubated with and without Phytohaemagglutinin (PHA).

ELISpot Plus: Bovine IL‑2 (ALP)

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT11A31)
Detection mAbMT3B3, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Bovine Interleukin-2 (IL-2) ELISpot. PBMC from cow incubated with and without Phytohaemagglutinin (PHA).
PBMC from cow were incubated in the presence or absence of Phytohaemagglutinin (PHA) and the number of cells secreting IL-2 was analyzed by bovine IL-2 ELISpot.

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.