ELISpot Plus: Human IL‑1α (ALP)

ELISpot Plus: Human IL‑1α (ALP)

3414-4APW-2

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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT641/642)
Detection mAbMT513, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-1α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-1α (ALP)
ApplicationELISpot
AnalyteIL-1α
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-1α (pro-form and active form).

Cross-reactivity

The assay shows no cross-reactivity with recombinant human IL-1β or IL-18.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC were incubated for 20 hours in an ELISpot plate. Cells were unstimulated (20,000 cells/well) or stimulated with LPS (5000 cells/well). The number of cells secreting IL-1α was analyzed by ELISpot.
Datasheet/Protocol
SDS

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Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-1α

Analyte description

Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation. 

Alternative namesInterleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
Cell typeMonocyte/MΦ, mDC
Gene ID3552