ELISpot Plus: Human IL-31 (ALP)

ELISpot Plus: Human IL‑31 (ALP)

3530-4APW-2

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Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT31/88)
Detection mAbMT158, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-31 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

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Product details

ProductELISpot Plus: Human IL-31 (ALP)
ApplicationELISpot
AnalyteIL-31
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-31.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-31 was analyzed by ELISpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-31

Analyte description

Interleukin 31 (IL-31) is a cytokine belonging to the IL-6 family. It is mainly secreted by activated T cells, especially in Th2 helper cells, mast cells, macrophages, and dendritic cells. IL-31 mainly acts in the skin, lung, intestine, and nervous system, facilitating cell-mediated immunity against pathogens. The receptor for IL-31 is found on e.g. keratinocytes and endothelial cells and can be upregulated on activated monocytes. However, IL-31 is considered a Th2-type cytokine with different functions than other Th2-type cytokines. In transgenic mice overexpressing IL-31, the cytokine induces pruritus and dermatitis resembling human atopic dermatitis (AD). In humans, IL-31 has been associated with AD, atopic and contact allergies, airway hypersensitivity, and inflammatory bowel disease. 
 

Alternative namesInterleukin-31, IL-31, IL31
Cell typeT cell, Tc, Th1, NK cell
Gene ID386653