ELISpot Plus: Mouse IL-12/-23 p40 (ALP)

ELISpot Plus: Mouse IL‑12/‑23 p40 (ALP)

3451-4APW-2

Publications: 0
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb C15.6)
Detection mAbC17.8, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

Low stock

Delivery within 3 weeks

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-12/-23 p40 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-12/-23 p40 (ALP)
ApplicationELISpot
AnalyteIL-12/-23 (p40)
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for the p40 subunit of mouse IL-12 and IL-23. The antibodies recognize p40 monomer and homodimer, IL-12 p70 (p40+p35), and IL-23 (p40+p19).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (200,000 cells/well) were incubated overnight in the absence or presence of LPS plus recombinant IFN-γ and the number of cells secreting IL-12/-23 (p40) was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
Loading publications...

IL-12/-23 (p40)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization.

Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.

Alternative namesInterleukin 12, IL-12, IL12, IL12A, IL12B, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16160