ELISpot Plus: Mouse IL‑12/‑23 p40 (ALP)

ELISpot Plus: Mouse IL‑12/‑23 p40 (ALP)

3451-4APW-2

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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb C15.6)
Detection mAbC17.8, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-12/-23 p40 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-12/-23 p40 (ALP)
ApplicationELISpot
AnalyteIL-12/-23 (p40)
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for the p40 subunit of mouse IL-12 and IL-23. The antibodies recognize p40 monomer and homodimer, IL-12 p70 (p40+p35), and IL-23 (p40+p19).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (200,000 cells/well) were incubated overnight in the absence or presence of LPS plus recombinant IFN-γ and the number of cells secreting IL-12/-23 (p40) was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IL-12/-23 (p40)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization.

Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.

Alternative namesInterleukin 12, IL-12, IL12, IL12A, IL12B, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16160