ELISpot Plus: Mouse IL‑1β (HRP)

ELISpot Plus: Mouse IL‑1β (HRP)

3317-4HPW-2

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Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MTB52)
Detection mAbMTB2433, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-1β using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-1β (HRP)
ApplicationELISpot
AnalyteIL-1β
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IL-1β.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

Mouse spleen cells (600,000 cells/well) were incubated overnight in the absence or presence of LPS and recombinant IFN-γ and the number of cells secreting IL-1β was analyzed by ELISpot.

Datasheet/ProtocolSDS

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Isolation, freezing, and thawing of splenocytes
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
 

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta
Cell typeMonocyte/MΦ, mDC
Gene ID16176