ELISpot Plus: Mouse TNF‑α (ALP)

ELISpot Plus: Mouse TNF‑α (ALP)

3511-4APW-2

Publications: 14
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT1C8/23C9)
Detection mAbMT11B10, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse TNF-α (ALP)
ApplicationELISpot
AnalyteTNF-α
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting TNF-alpha (TNF-α) was analyzed by ELISpot.
Datasheet/ProtocolSDS

Highlighted research, Published October 11, 2024

Immunoassays for vaccine research
Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID21926