ELISpot substrate: BCIP/NBT‑plus for ALP

3650-10

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Product specifications

Intended use

This ready-to-use BCIP/NBT-plus substrate is intended for the development of ELISpot assays using Streptavidin-ALP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Adjust to room temperature before use. Filter the substrate solution through a 0.45 µm filter and add to ELISpot plates after incubation with alkaline phosphatase and subsequent washing. Develop until spots emerge. Stop the colour development by washing extensively in tap water.

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