Anti-CD28 mAb (CD28-A)

FluoroSpot enhancer

FluoroSpot plates 

FluoroSpot Flex pack 1

R848

Recombinant human IL-2

FluoroSpot Flex pack 2

FluoroSpot Flex pack 3

Anti-CD28 mAb 

FluoroSpot Flex pack 4

Recombinant mouse IL-2

FluoroSpot Flex pack 5

FluoroSpot Flex pack 6

FluoroSpot Flex pack 7

FluoroSpot Plus: Human IFN-γ/IL-10

FluoroSpot Plus: Human IFN-γ/IL-22/IL-17A

FluoroSpot Plus: Human IFN-γ/IL-10/IL-17A

FluoroSpot Plus: Human IFN-γ/IL-5

FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α

FluoroSpot Plus: Human IFN-γ/TNF-α

FluoroSpot Plus: Human IFN-γ/IL-17A/IL-5

FluoroSpot Plus: Human IFN-γ/IL-10/IL-5

FluoroSpot Plus: Monkey IFN-γ/IL-2

FluoroSpot Plus: Human IFN-γ/IL-13

FluoroSpot Plus: Mouse IFN-γ/IL-10

FluoroSpot Plus: Mouse IFN-γ/IL-10/IL-5

FluoroSpot Plus: Mouse IFN-γ/IL-17A/IL-5

FluoroSpot Plus: Human IFN-γ/IL-10/IL-2

FluoroSpot Plus: Human IL-22/IFN-γ/IL-10/IL-17A

FluoroSpot Plus: Human IL-22/IFN-γ/IL-5/IL-17A

FluoroSpot Plus: Human IL-1β/IL-6/TNF-α

FluoroSpot Plus: Human IFN-γ (490)

FluoroSpot Plus: Human IFN-γ (550)

FluoroSpot Plus: Mouse IFN-γ (490)

FluoroSpot Plus: Mouse IFN-γ (550)

FluoroSpot Plus: Monkey IFN-γ (550)

FluoroSpot Plus: Mouse IFN-γ/IL-4

FluoroSpot Plus: Human IFN-γ/IL-4

FluoroSpot Plus: Mouse IFN-γ/IL-2

FluoroSpot Plus: Human IL-2 (550)

FluoroSpot Plus: Mouse IL-2 (550)

FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/IL-2 

FluoroSpot Plus: Monkey IFN-γ/IL-17A

FluoroSpot Plus: Monkey IFN-γ/TNF-α

FluoroSpot Plus: Mouse IFN-γ/IL-17A 

FluoroSpot Plus: Monkey IFN-γ/TNF-α/IL-2

FluoroSpot Plus: Human IL-6/IL-1β/GM-CSF/TNF-α 

FluoroSpot Path: SARS-CoV-2 (RBD) Human IgG 

FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B 

FluoroSpot Plus: Human IFN-γ/Granzyme B

FluoroSpot Plus: Human IFN-γ/Granzyme B/IL-2 

FluoroSpot Plus: Human IFN-γ/Granzyme B/TNF-α 

FluoroSpot Plus: Human IFN-γ/IL-10/Granzyme B

FluoroSpot Path: SARS-CoV-2 (Spike) Human IgG

FluoroSpot Path: SARS-CoV-2 (Spike+RBD) Human IgG

FluoroSpot Path: SARS-CoV-2 (S+NMO) Human IFN-γ/IL-2 

FluoroSpot Path: CMV Human IFN-γ/IL-2

FluoroSpot Path: AdV5 (hexon) Human IFN-γ/IL-2 

FluoroSpot Plus: Human IFN-γ/IL-4/IL-5

FluoroSpot Plus: Mouse IFN-γ/TNF-α

FluoroSpot Plus: Mouse IFN-γ/IL-2/TNF-α 

FluoroSpot Plus: Mouse IFN-γ/IL-5 

FluoroSpot Path: TB (ESAT-6+CFP-10+EspC) Human IFN-γ/IL-2 

FluoroSpot Plus: Human IFN-γ/IL-2

FluoroSpot Path: SARS-CoV-2 (S+NMO) Human IFN-γ/Granzyme B/IL-2

FluoroSpot Path: SARS-CoV-2 (Omicron BA.1, S1 scan) Human IFN-γ/IL-2 

FluoroSpot Plus: Human IFN-γ/IL-4/TNF-α

FluoroSpot Plus: Human IFN-γ/IL-5/TNF-α

FluoroSpot Plus: Mouse IFN-γ/IL-10/IL-17A 

FluoroSpot Plus: Mouse IFN-γ/IL-22/IL-17A

FluoroSpot Path: EBV Human IFN-γ/IL-2

Capture mAb (1-D1K)

BAM-conjugated detection mAb (7-B6-1)

Anti-BAM-490

A FluoroSpot reader equipped with a bandpass filter for excitation at 490 nm and emission at 510 nm (e.g., LED490, FITC-equivalent) is recommended for analysis.

Human IFN-γ FluoroSpot. Human PBMC were incubated with CEF peptide pool and anti-CD28 antibody (both available from Mabtech). Spots from IFN-γ secreting cells were analyzed with Mabtech IRIS.

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Human IFN-γ FluoroSpot Flex set (490)

Biotinylated detection mAb (7-B6-1)

SA-550

A FluoroSpot reader equipped with a bandpass filter for excitation at 550 nm and emission at 570 nm (e.g., LED550, Cy3-equivalent) is recommended for analysis.

Human IFN-γ FluoroSpot. Human PBMC were incubated with anti-CD3 and anti-CD28 antibody. Spots from IFN-γ secreting cells were analyzed with Mabtech IRIS.

human-ifng-550-fluorospot.png

Human IFN-γ FluoroSpot Flex set (550)

DIG-conjugated detection mAb (7-B6-1)

Anti-DIG-380

A FluoroSpot reader equipped with a bandpass filter for excitation at 380 nm and emission at 430 nm (e.g., LED380) is recommended for analysis.

Human IFN-γ FluoroSpot. Human PBMC (50,000 cells/well) incubated with PHA overnight. Spots from IFN-γ secreting cells were analyzed with Mabtech IRIS.

human-ifng-380-fluorospot.png

Human IFN-γ FluoroSpot Flex set (380)

WASP-conjugated detection mAb (7-B6-1)

Anti-WASP-640

A FluoroSpot reader equipped with a bandpass filter for excitation at 640 nm and emission at 660 nm (e.g., LED640, Cy5-equivalent) is recommended for analysis.

human-ifng-640-fluorospot.png

Human IFN-γ FluoroSpot Flex set (640)

Capture mAbs (MT2A91/2C95)

BAM-conjugated detection mAb (MT8G10)

Human IL-2 FluoroSpot (490). Human PBMC (50,000 cells/well) stimulated overnight with PHA and anti-CD28 mAb. Spots from IL-2 secreting cells were analyzed with Mabtech IRIS.

human-il2-490-fluorospot.png

Human IL-2 FluoroSpot Flex set (490)

Biotinylated detection mAb (MT8G10)   

For analysis we recommend the use of an automated FluoroSpot reader equipped with a LED550 filter, excitation 550 nm/emission 570 nm (i.e. Cy3 equivalent).

Human IL-2 FluoroSpot (550). Human PBMC (50,000 cells/well) stimulated overnight with PHA and anti-CD28 mAb. Spots from IL-2 secreting cells were analyzed with Mabtech IRIS. 

human-il2-550-fluorospot.png

Human IL-2 FluoroSpot Flex set (550)

DIG-conjugated detection mAb (MT8G10)

Human IL-2 FluoroSpot (380). Human PBMC (50,000 cells/well) stimulated overnight with PHA and anti-CD28 mAb. Spots from IL-2 secreting cells were analyzed with Mabtech IRIS.

human-il2-380-fluorospot.png

Human IL-2 FluoroSpot Flex set (380)

WASP-conjugated detection mAb (MT8G10) 

Human IL-2 FluoroSpot (640). Human PBMC (50,000 cells/well) stimulated overnight with PHA and anti-CD28 mAb. Spots from IL-2 secreting cells were analyzed with Mabtech IRIS. 

human-il2-640-fluorospot.png

Human IL-2 FluoroSpot Flex set (640)

Capture mAb (MT44.6)

BAM-conjugated detection mAb (MT504) 

Human IL-17A FluoroSpot. Human PBMC were incubated with Candida albicans extract and anti-CD28 antibody. Spots from IL-17A secreting cells were analyzed with Mabtech IRIS.

human-il17a-490-fluorospot.png

Human IL-17A FluoroSpot Flex set (490)

Biotinylated detection mAb (MT504)

Human IL-17A FluoroSpot. Human PBMC were incubated with Candida albicans extract. Spots from IL-17A secreting cells were analyzed with Mabtech IRIS.

human-il17a-550-fluorospot.png

Human IL-17A FluoroSpot Flex set (550)

WASP-conjugated detection mAb (MT504) 

Human IL-17A FluoroSpot. Human PBMC were incubated with PHA. Spots from IL-17A secreting cells were analyzed with Mabtech IRIS.

human-il17a-640-fluorospot.png

Human IL-17A FluoroSpot Flex set (640)

Capture mAb (MT1318)

BAM-conjugated detection mAb (25K2)

Human IL-13 FluoroSpot. Human PBMC (100,000 cells/well) were incubated with PHA for 48 hours. Spots from IL-13 secreting cells were analyzed with Mabtech IRIS.

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Human IL-13 FluoroSpot Flex set (490)

Biotinylated detection mAb (25K2)

human-il13-550-fluorospot.png

Human IL-13 FluoroSpot Flex set (550)

DIG-conjugated detection mAb (25K2)

human-il13-380-fluorospot.png

Human IL-13 FluoroSpot Flex set (380)

WASP-conjugated detection mAb (25K2)

human-il13-640-fluorospot.png

Human IL-13 FluoroSpot Flex set (640)

Capture mAbs (MT91/145)

Detection mAbs anti-human IgG-490 (MT78/145)

Human IgG FluoroSpot. Human PBMC pre-stimulated with R848 and recombinant human IL-2. Spots from IgG secreting cells were analyzed with Mabtech IRIS.  

human-igg-490-fluorospot.png

Human IgG FluoroSpot Flex set (490)

Detection mAbs anti-human IgG-550 (MT78/145)

Human IgG FluoroSpot. Human PBMC pre-stimulated with R848 and recombinant human IL-2. Spots from IgG secreting cells were analyzed with Mabtech IRIS.

human-igg-550-fluorospot.png

Human IgG FluoroSpot Flex set (550)

Capture mAb (MT57)

Detection mAb anti-human IgA-490 (MT20) 

Human IgA FluoroSpot. Human PBMC pre-stimulated with R848 and recombinant human IL-2. Spots from IgA secreting cells were analyzed with Mabtech IRIS.

human-iga-490-fluorospot.png

Human IgA FluoroSpot Flex set (490)

Capture mAb (9D7)

Biotinylated detection mAb (12G8)

Human IL-10 FluoroSpot. Human PBMC were incubated with the CEF peptide pool and anti-CD28 antibody (both available from Mabtech). Spots from IL-10 secreting cells were analyzed with Mabtech IRIS.

human-il10-550-fluorospot.png

Human IL-10 FluoroSpot Flex set (550)

Capture mAb (TRFK5)

Biotinylated detection mAb (5A10)

Human IL-5 FluoroSpot. Human PBMC were incubated with PHA. Spots from IL-5 secreting cells were analyzed with Mabtech IRIS.

human-il5-550-fluorospot.png

Human IL-5 FluoroSpot Flex set (550)

WASP-conjugated detection mAb (5A10)

Human IL-5 FluoroSpot. Human PBMC were incubated with PPD and anti-CD28 antibody. Spots from IL-5 secreting cells were analyzed with Mabtech IRIS.

human-il5-640-fluorospot.png

Human IL-5 FluoroSpot Flex set (640)

Capture mAb (MT25C5)

BAM-conjugated detection mAb (MT20D9)

Human TNF-α FluoroSpot. Human PBMC were pre-incubated with R848. Spots from TNF-α secreting cells were analyzed with Mabtech IRIS. 

human-tnfa-490-fluorospot.png

Human TNF-α FluoroSpot Flex set (490)

Biotinylated detection mAb (MT20D9)

Human TNF-α FluoroSpot. Human PBMC were incubated with LPS. Spots from TNF-α secreting cells were analyzed with Mabtech IRIS. 

human-tnfa-550-fluorospot.png

Human TNF-α FluoroSpot Flex set (550)

DIG-conjugated detection mAb (MT20D9) 

Human TNF-α FluoroSpot. Human PBMC (5000 cells/well) were stimulated with LPS. Spots from TNF-α secreting cells were analyzed with Mabtech IRIS.  

human-tnfa-380-fluorospot.png

Human TNF-α FluoroSpot Flex set (380)

WASP-conjugated detection mAb (MT20D9)

human-tnfa-640-fluorospot.png

Human TNF-α FluoroSpot Flex set (640)

Capture mAbs (Pf-80/164)

Biotinylated detection mAb (Pf-344)

Human Perforin FluoroSpot (550). Human PBMC were stimulated with anti-CD3 antibody overnight. Spots from Perforin secreting cells were analyzed with Mabtech IRIS.

human-perforin-550-fluorospot.png

Human Perforin FluoroSpot Flex set (550)

Capture mAb (MT175)

BAM-conjugated detection mAb (7P10)

Human IL-1β FluoroSpot (490). Human PBMC (15,000 cells/well) stimulated overnight with LPS. Spots from IL-1β secreting cells were analyzed with Mabtech IRIS.

human-il1b-490-fluorospot.png

Human IL-1β FluoroSpot Flex set (490)

Biotinylated detection mAb (7P10)

Human IL-1β FluoroSpot (550). Human PBMC (15,000 cells/well) stimulated overnight with LPS. Spots from IL-1β secreting cells were analyzed with Mabtech IRIS.

human-il1b-550-fluorospot.png

Human IL-1β FluoroSpot Flex set (550)

DIG-conjugated detection mAb (7P10)

Human IL-1β FluoroSpot (380). Human PBMC (15,000 cells/well) stimulated overnight with LPS. Spots from IL-1β secreting cells were analyzed with Mabtech IRIS.

human-il1b-380-fluorospot.png

Human IL-1β FluoroSpot Flex set (380)

FluoroSpot Flex

Reactivity

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Your FluoroSpot Flex kit