FluoroSpot Path: SARS‑CoV‑2 (Spike) Human IgG

FSP-05R-S1-1

Publications: 0

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Bulk or custom format

Paves your way to functional analysis of a specific research question.

Components

Plate	Pre-coated FluoroSpot plate (mAbs MT91/145)
Detection mAb	Anti-IgG mAbs (MT78/145), 550
Antigen	Spike-GAL
Fluorophore conjugate	Anti-GAL mAb, 490
Buffers/Solutions	FluoroSpot enhancer
	Reconstitution buffer
Stimuli	R848
	Recombinant human IL-2

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Path kit is intended for the enumeration of B cells secreting human IgG in response to SARS-CoV-2 (Spike) using the FluoroSpot assay. B cells secreting IgG irrespective of antigen specificity (total IgG) can also be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).

Recommendation

Product details

Product	FluoroSpot Path: SARS-CoV-2 (Spike) Human IgG
Application	FluoroSpot
Analyte	IgG
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for the Fc part of human IgG.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Recombinant protein should be stored at -20 °C or below. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Human PBMC from a COVID-19 convalescent individual were incubated with R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an FluoroSpot plate (250,000 cells/well) coated with anti-human IgG mAbs. Spike-specific IgG spots were detected with Spike-GAL, i.e., recombinant Spike protein with a GAL peptide tag, followed by anti-GAL-490. Spots were analyzed with Mabtech IRIS.

Documents

Datasheet/Protocol SDS

Product highlights

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research

Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research, Published December 16, 2021

SARS-CoV-2-specific immune responses in patients with autoimmune diseases

Patients with rheumatic diseases showed T cell responses comparable to healthy control. Patients with MS and after mRNA vaccination show different memory B cell IgG responses. SARS-CoV-2 specific immune responses in autoimmunity was analyzed using FluoroSpot, ELISpot Path, and Mabtech IRIS.

Highlighted research, Published December 2, 2021

Importance of studying SARS-CoV-2-specific memory B cells

When studying antibodies in circulation, remember that memory B cells might hide out of sight. In this study, detection of SARS-CoV-2-specific T and memory B cells 6 and 15 months after infection.

Highlighted research, Published November 24, 2021

Polyfunctional T cells with Mabtech IRIS

Study of development and persistence of virus-specific cellular and humoral immune responses, during the acute phase and two follow-ups after COVID-19 disease in hospitalized patients. Mabtech’s FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α were analyzed with Mabtech IRIS.

Highlighted research, Published May 30, 2020

B cell FluoroSpot for sensitive ASC detection

Mabtech IRIS and IgG/IgA/IgM FluoroSpot were used to study antibody-secreting plasma cells (ASCs) specific for the nucleocapsid (N) protein. FluoroSpot was shown to be more sensitive than e.g., ELISA.

Tutorials

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (?)

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Analyte information

IgG

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Alternative names	Immunoglobulin G, IgG, IgG3
Cell type	B cell
Gene ID	3500, 3501, 3502