FluoroSpot Plus: Human IFN‑γ/TNF‑α

FSP-0109-2

Publications: 3

Documents

Bulk or custom format

Ensure reproducibility when diving into a multiplex analysis.

Components

Plate	Pre-coated FluoroSpot plate (mAbs 1-D1K and MT25C5)
Detection mAbs	Anti-IFN-γ mAb (7-B6-1), BAM
	Anti-TNF-α mAb (MT20D9), biotin
Fluorophore conjugates	Anti-BAM mAb, 490
	SA-550
Buffer/Solution	FluoroSpot enhancer
Positive control	Anti-CD3 mAb (CD3-2)
Co-stimulus	Anti-CD28 mAb (CD28-A)

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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PepPool: CEF (CD8), human

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and TNF-α using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Human IFN-γ/TNF-α
Application	FluoroSpot
Analyte	IFN-γ, TNF-α
Reactivity	Human
Specificity	This kit is based on matched pairs of mAbs specific for human IFN-γ and TNF-α.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the CEF peptide pool and anti-CD28 antibody (both available from Mabtech) and the number of cells secreting IFN-γ and/or TNF-α were analyzed by FluoroSpot. Double-secreting cells were determined as spots having the same center point in an image overlay of LED490, FITC (IFN-γ) and LED550, Cy3 (TNF-α) images. Spot analysis was made with Mabtech IRIS.

Documents

Datasheet/Protocol SDS

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Isolation, freezing, and thawing of PBMCs

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Publications (3)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124