FluoroSpot Plus: Mouse IFN-γ/IL-10

FluoroSpot Plus: Mouse IFN‑γ/IL‑10

FSP-4147-2

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Components

PlatePre-coated FluoroSpot plate (mAbs AN18 and MT60)
Detection mAbsAnti-IFN-γ mAb (R4-6A2), BAM
Anti-IL-10 mAb (51E6), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Buffer/SolutionFluoroSpot enhancer
Co-stimulusAnti-CD28 mAb

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-10 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Mouse IFN-γ/IL-10
ApplicationFluoroSpot
AnalyteIFN-γ, IL-10
ReactivityMouse
SpecificityThis kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-10.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Mouse spleen cells were incubated overnight in the absence or presence of Concanavalin A (ConA) and anti-CD28 antibody. The number of cells secreting IFN-γ and/or IL-10 was analyzed by FluoroSpot. Double secreting cells were determined as spots having the same center point in an image overlay. Spot analysis was made with Mabtech IRIS.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978

IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, If2a, Il-10
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID16153
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