FluoroSpot Plus: Mouse IFN-γ/IL-5

FluoroSpot Plus: Mouse IFN‑γ/IL‑5

FSP-4143-2

Publications: 6

Documents

Questions?

Ensure reproducibility when diving into a multiplex analysis.

Components

Plate	Pre-coated FluoroSpot plate (mAbs AN18 and TRFK5)
Detection mAbs	Anti-IFN-γ mAb (R4-6A2), BAM
	Anti-IL-5 mAb (TRFK4), biotin
Fluorophore conjugates	Anti-BAM mAb, 490
	SA-550
Buffer/Solution	FluoroSpot enhancer
Co-stimulus	Anti-CD28 mAb

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ and IL-5 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Mouse IFN-γ/IL-5
Application	FluoroSpot
Analyte	IFN-γ, IL-5
Reactivity	Mouse
Specificity	This kit is based on matched pairs of mAbs specific for mouse IFN-γ and IL-5.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Mouse spleen cells (200,000 cells/well) were incubated for 20 hours in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ and/or IL-5 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550,Cy3 (IL-5). Spot analysis was made with Mabtech IRIS.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (6)

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Analyte information

IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	15978

IL-5

Analyte description	Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.
Alternative names	Interleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell type	T cell, Th2, NK cell
Gene ID	16191