FociSpot Path: Influenza A (NP)

FociSpot Path: Influenza A (NP)

4405-4AT-1

Publications: 0
Documents
Questions?
Quantifying foci has never been easier.

Content

Plate96-well tissue culture treated plate
Detection mAb19C10, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This kit is intended for FociSpot immunostaining of the influenza A nucleoprotein, which enables enumeration of influenza A virus foci in focus forming assays (FFA). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

FociSpot
In FociSpot, virus foci are detected by using mAbs specific to viral proteins. After fixation and permeabilization of infected cells in a 96-well plate, the virus-specific biotinylated detection mAb is added. The use of Streptavidin-ALP enzyme conjugate, followed by a precipitating substrate enables detection of foci. Foci can be counted manually, but counting with Mabtech IRIS 2 is rapid and offers easy data handling.

Recommendation

Product details

ProductFociSpot Path: Influenza A (NP)
ApplicationFociSpot
AnalyteInfluenza A (NP)
Specificity

Influenza A nucleoprotein (NP)

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibody, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of influenza A nucleoprotein in infected MDCK cells. MDCK cells were infected with influenza A virus (titrated, virus dilutions 2,000x, 4,000x, and 8,000x are shown). After fixation and permeabilization, foci were detected using anti-influenza A (NP) mAb 19C10, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Featured product, Published October 5, 2023

Influenza A FociSpot kit and monoclonal antibody
Measure the amount of infectious particles and determine the levels of neutralizing antibodies.

Tutorial, Published October 12, 2023

Step-by-step guide to FociSpot
FociSpot is our new assay platform that includes both kits and a brand-new analysis capability of Mabtech IRIS™ 2 allowing for automated counting of foci in a 96-well plate format. Let us show you how you can run an FRNT50 with FociSpot.
Loading publications...

Influenza A (NP)

Analyte description

The Nucleoprotein (NP) is a structural protein and encapsidates the negative strand RNA of influenza A into ribonucleoproteins (RNP). NP plays a role in replication, transcription, RNA packaging, and nuclear trafficking. The NP does not only interact with the RNA, but also the viral polymerase, the M protein, and several host factors. 

Alternative namesNucleoprotein, NP (of influenza A)