FociSpot Path: SARS-CoV-2 (N)

FociSpot Path: SARS‑CoV‑2 (N)

4401-4AT-1

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Content

Plate96-well tissue culture treated plate
Detection mAbMTCC25, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This kit is intended for FociSpot immunostaining of the SARS-CoV-2 nucleocapsid (N) protein, which enables enumeration of SARS-CoV-2 virus foci in Focus Forming Assays (FFA). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

FociSpot
In FociSpot, virus foci are detected by using mAbs specific to viral proteins. After fixation and permeabilization of infected cells in a 96-well plate, the virus-specific biotinylated detection mAb is added. The use of Streptavidin-ALP enzyme conjugate, followed by a precipitating substrate enables detection of foci. Foci can be counted manually, but counting with Mabtech IRIS 2 is rapid and offers easy data handling.

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Product details

ProductFociSpot Path: SARS-CoV-2 (N)
ApplicationFociSpot
AnalyteSARS-CoV-2 (N)
Specificity

SARS-CoV-2 nucleocleocapsid

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibody, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS

Tutorial, Published October 12, 2023

Step-by-step guide to FociSpot
FociSpot is our new assay platform that includes both kits and a brand-new analysis capability of Mabtech IRIS™ 2 allowing for automated counting of foci in a 96-well plate format. Let us show you how you can run an FRNT50 with FociSpot.

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.
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SARS-CoV-2 (N)

Analyte description

The nucleocapsid (N) protein is a structural protein of the SARS-CoV-2 virus. It is responsible for packaging the positive-sense RNA genome by forming ribonucleoprotein (RNP) structures with the viral RNA. The N protein is mainly expressed in the host cells' cytoplasm and interacts with the M protein during assembly. The nucleocapsid protein is highly immunogenic and antibodies against it are found in people that recovered from COVID-19. The N protein has been suggested to suppress type I interferon responses. 

Alternative namesNucleocapsid protein, N protein (of SARS-CoV-2)