FluoroSpot Plus: Human IFN-γ/IL-5/TNF-α
FluoroSpot Plus: Human IFN‑γ/IL‑5/TNF‑α
Components
| Plate | Pre-coated FluoroSpot plate (mAbs 1-D1K, TRFK5, and MT25C5) |
| Detection mAbs | Anti-IFN-γ mAb (7-B6-1), BAM |
| Anti-IL-5 mAb (5A10), biotin | |
| Anti-TNF-α mAb (MT20D9), WASP | |
| Fluorophore conjugates | Anti-BAM mAb, 490 |
| SA-550 | |
| Anti-WASP mAb, 640 | |
| Buffer/Solution | FluoroSpot enhancer |
| Positive control | Anti-CD3 mAb (CD3-2) |
| Co-stimulus | Anti-CD28 mAb (CD28-A) |
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Complementary products
Complementary products
Performance
Human PBMC (250,000 cells/well) were stimulated with a mixture of CEF extended, CMV, and CEFTA peptide pools (Mabtech) and anti-CD28 for 42 h and the number of cells secreting IFN-γ, IL-5 and TNF-α were analyzed by FluoroSpot using Mabtech IRIS.
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 3458 |
IL-5
| Analyte description | Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells. |
| Alternative names | Interleukin-5, IL-5, IL5, BCGF-2, EDF, TRF |
| Cell type | T cell, Th2, NK cell |
| Gene ID | 3567 |
TNF-α
| Analyte description | Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock. |
| Alternative names | Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F |
| Cell type | T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ |
| Gene ID | 7124 |