Goat anti-human IgG, ALP

3310-1-1000

Publications: 85

Documents

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Product specifications

Intended use

This ALP-conjugated antibody enables specific detection of human IgG in enzyme immunoassays such as indirect ELISA and Western blot.

Serum/Plasma samples

Recommendation

Dilute 1/1000 as a general guideline. Dilution factor may vary with differing assay conditions.

Product details

Product	Goat anti-human IgG, ALP
Application	ELISA, Western blot
Analyte	IgG
Antibody	Goat anti-human IgG
Conjugate	ALP
Clonality	Polyclonal
Immunogen	Native human IgG
Host	Goat
Reactivity	Human
Specificity	Human IgG H-chains (Fcγ)
Cross-reactivity	No reactivity with human IgA, IgM, or mouse Ig. May cross-react with IgG from other species.
Supplied in	0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG.
Contents	Goat anti-human IgG, ALP-conjugated. Supplied in 0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol SDS

Human IgG mAb guide

Learn how to combine Mabtech’s antibodies for human IgG ELISA and ELISpot. Adjust the setup to detect either total IgG or antigen-specific IgG. Different antibody combinations of monoclonal antibodies have been selected for the detection of the human IgG subclasses in ELISA and ELISpot. There is no cross-reactivity to human IgA1, IgA2, IgM, and IgE.

Application guide: ELISA

The human IgG ELISA can be adapted to detect antigen-specific IgG or the total IgG in serum or plasma samples.

Antigen-specific IgG

Capture: Antigen
Detection mAbs: MT78/145, biotin or MT78, ALP

Total IgG

Capture mAb: MT145, unconjugated
Detection mAb: MT78, ALP

Application guide: ELISpot

The human IgG ELISpot and FluoroSpot can be adapted to detect antigen-specific IgG-secreting cells or the total IgG-secreting cells in cell samples like PBMCs.

Antigen-specific IgG

Capture:
Antigen
Detection mAbs:
MT78/145, biotin

Capture mAbs:
MT91/145, unconjugated
Detection:
Antigen-biotin

Total IgG

Capture mAbs:
MT91/145, unconjugated
Detection mAbs:
MT78/145, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgG ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database!

Publications (85)

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Analyte information

IgG

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Alternative names	Immunoglobulin G, IgG, IgG3
Cell type	B cell
Gene ID	3500, 3501, 3502