ELISA Flex: Human Granzyme A (HRP)

Standard curve for human Granzyme A ELISA Flex kit.

ELISA Flex: Human Granzyme A (HRP)

3484-1H-6

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You may add these complementary products at a reduced price.

ELISA diluent
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ELISA substrate: TMB for HRP
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This offer is valid when purchasing ELISA Flex: Human Granzyme A (HRP) or other qualifying products.

Publications: 4
Documents
Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbGA29
Detection mAbGA28, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate
StandardRecombinant human granzyme A ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human granzyme A in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human Granzyme A (HRP)
ApplicationELISA
AnalyteGranzyme A
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human granzyme A.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range125-12500 pg/ml
Standard curve for human Granzyme A ELISA Flex kit.
Representative standard curve for human Granzyme A ELISA Flex kit using TMB substrate.
Datasheet/ProtocolSDS

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorial, Published April 8, 2025

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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Granzyme A

Analyte description

Granzyme A is a serine protease packed together with e.g., granzyme B and perforin in cytoplasmic granules within cytotoxic T cells and NK cells. Release of the granule content is crucial for the elimination of infected or transformed cells. Perforin facilitates the delivery of granzymes, and cells resistant to the cytotoxic action of granzyme B may be sensitive to granzyme A and vice versa. Thus, the content of the granules will act in concert to induce target cell death by apoptosis.

Alternative namesGranzyme A, GzmA, GrA, GrzA, CTLA3, HFSP
Cell typeT cell, Tc, Th1, Th17, NK cell
Gene ID3001