FluoroSpot Plus: Human IFN-γ/IL-13
FluoroSpot Plus: Human IFN‑γ/IL‑13
Components
| Plate | Pre-coated FluoroSpot plate (mAbs 1-D1K and MT1318) |
| Detection mAbs | Anti-IFN-γ mAb (7-B6-1), BAM |
| Anti-IL-13 mAb (25K2), biotin | |
| Fluorophore conjugates | Anti-BAM mAb, 490 |
| SA-550 | |
| Buffer/Solution | FluoroSpot enhancer |
| Positive control | Anti-CD3 mAb (CD3-2) |
| Co-stimulus | Anti-CD28 mAb (CD28-A) |
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 3458 |
IL-13
| Analyte description | Interleukin 13 (IL-13) is produced by Th2-type cells, mast cells, and NK cells in response to activation. It regulates the secretion of IgE by B cells and the Th2-type cell development and suppresses inflammatory responses by regulation of macrophage function. |
| Alternative names | Interleukin 13, IL-13, IL13, P600 |
| Cell type | T cell, Th2, Treg |
| Gene ID | 3596 |