ELISA Pro: Human IgE

3810-1HP-1

Publications: 18

Documents

Bulk or custom format

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb 107)
Detection mAb	182, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Native human IgE ELISA standard
Substrate	ELISA substrate: TMB for HRP
Buffers/Solutions	ELISA diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IgE in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human IgE
Application	ELISA
Analyte	IgE
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	0.1-31.6 ng/ml
Sensitivity	0.03 ng/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 5 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human IgE ELISA Pro kit.

Documents

Datasheet/Protocol SDS

Human IgE mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

Application guide: ELISA

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

Application guide: ELISpot

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

How to detect immunoglobulins with ELISA

Detect and quantify immunoglobulins in solution, either antigen-specific or even total Ig.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Publications (18)

Loading publications...

Analyte information

IgE

Analyte description	The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.
Alternative names	Immunoglobulin E, IgE
Cell type	B cell
Gene ID	3497