ELISA Pro: Human Thioredoxin-1

ELISA Pro: Human Thioredoxin‑1

3580-1HP-1

Publications: 5

Documents

Questions?

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb MT17R6)
Detection mAb	MT13X3, biotin
Enzyme conjugate	Streptavidin-HRP
Standard	Recombinant human thioredoxin-1 ELISA standard
Substrate	ELISA substrate: TMB for HRP
Buffers/Solutions	Assay buffer
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

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Delivery within 3 weeks

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of reduced and oxidized forms of human Thioredoxin-1 (Trx1) in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human Thioredoxin-1
Application	ELISA
Analyte	Thioredoxin-1
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human Thioredoxin-1 (Trx1). Reduced and oxidized forms of Trx1 are detected.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	31.2-2000 pg/ml
Sensitivity	15 pg/ml The limit of detection is the lowest concentration that is possible to detect but not necessarily quantify with precision and accuracy.

Representative standard curve for human Thioredoxin-1 (Trx1) ELISA Pro kit using TMB substrate.

Documents

Datasheet/Protocol
SDS

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Publications (5)

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Analyte information

Thioredoxin-1

Analyte description	Thioredoxin-1 (Trx1) is an oxidoreductase protein and thus may act as an anti-oxidant by facilitating the reduction of cysteine residues of other proteins. Besides keeping the intracellular milieu in a reduced state and being involved in the defence against oxidative stress, thioredoxin-1 specifically regulates several transcription factors and receptors. Increased plasma levels of thioredoxin-1 has been associated with several psychiatric disorders.
Alternative names	Thioredoxin-1, Trx1, TRDX, TRX, TRX1, Trx80
Cell type	T cell, Tc, Th1, Th2, Th17, Treg, Monocyte/MΦ
Gene ID	7295