ELISpot Plus: Mouse IL-1α (HRP)

ELISpot Plus: Mouse IL‑1α (HRP)

3417-4HPW-2

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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT946)
Detection mAbMT951, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-1α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-1α (HRP)
ApplicationELISpot
AnalyteIL-1α
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IL-1α.

Cross-reactivity

The mAbs do not cross-react with mouse IL-1β.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells were incubated for 20 hours in an ELISpot plate. Cells were unstimulated or stimulated with LPS (200,000 cells/well). The number of cells secreting IL-1α was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 7, 2022

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DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

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Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-1α

Analyte description

Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation. 

Alternative namesInterleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, Il-1a
Cell typeMonocyte/MΦ, mDC
Gene ID16175