ELISpot Plus: Mouse IL-10 (HRP)

ELISpot Plus: Mouse IL‑10 (HRP)

3432-4HPW-2

Publications: 12
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT60)
Detection mAb51E6, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-10 (HRP)
ApplicationELISpot
AnalyteIL-10
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-10.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells were incubated overnight in the presence (50,000 cells/well) or absence (100,000 cells/well) of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, If2a, Il-10
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID16153
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