ELISpot Plus: Mouse IL‑10 (HRP)

Mouse spleen cells were incubated overnight with or without LPS and the number of cells secreting IL-10 was analyzed by ELISpot.

ELISpot Plus: Mouse IL‑10 (HRP)

3432-4HPW-2

Publications: 13
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT60)
Detection mAb51E6, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-10 (HRP)
ApplicationELISpot
AnalyteIL-10
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-10.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells were incubated overnight with or without LPS and the number of cells secreting IL-10 was analyzed by ELISpot.
Mouse spleen cells were incubated overnight in the presence (50,000 cells/well) or absence (100,000 cells/well) of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.
Mouse spleen cells were incubated overnight with or without LPS and the number of cells secreting IL-10 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, If2a, Il-10
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID16153