ELISpot Plus: Mouse IL-5 (ALP)

ELISpot Plus: Mouse IL‑5 (ALP)

3391-4APW-2

Publications: 15

Documents

Questions?

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb TRFK5)
Detection mAb	TRFK4, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Mouse IL-5 (ALP)
Application	ELISpot
Analyte	IL-5
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IL-5.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Mouse spleen cells (400,000 cells/well) were incubated for 40h in the absence or presence of Concanavalin A (ConA) plus anti-CD28 antibody and the number of cells secreting IL-5 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot

An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (15)

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Analyte information

IL-5

Analyte description	Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.
Alternative names	Interleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell type	T cell, Th2, NK cell
Gene ID	16191