Peptide pool with 65 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).
In stock
Delivery 4-9 business days
Shipping $0
Product specifications
Intended use
This peptide pool is intended for studying immune responses against BK polyomvirus (BKV) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
Description
This peptide pool induces secretion of e.g., IFN-γ from human CD4 and CD8 T cells. It contains 65 synthetic peptides derived from the BKV large T protein, small T protein, and VP1 protein. 50 of the peptides are MHC class I-restricted and 15 are MHC class II-restricted. The peptide pool is designed to stimulate T cells with a broad array of HLA class I supertypes. Sequence similarity to corresponding JC virus (JCV) peptides ranges from 60% to 100%, with a median sequence similarity of 80%.
Recommendation
Product details
Product
PepPool: BKV (CD4 and CD8), human
Application
T cell stimulation, ELISpot, FluoroSpot
Reactivity
Human
Concentration
One vial with 25 µg of each peptide
Supplied in
Lyophilized
Contents
Peptide pool with 65 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).
Shipping and Storage
Shipping
Shipped at ambient temperature.
Storage
Store at -20 °C or below upon receipt.
Performance
Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: BKV (CD4 + CD8), human, and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS.