PepPool: CyCMV+RhCMV (TP‑UL83+Rh112), scanning

PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.

PBMC from rhesus macaque (300,000 cells/well) were incubated overnight in the absence or presence of the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ FluoroSpot. Analysis was done with Mabtech IRIS.

PepPool: CyCMV+RhCMV (TP‑UL83+Rh112), scanning

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Apo ELISA buffer

Product specifications

Intended use

This peptide pool is intended for studying immune responses in rhesus and cynomolgus macaques against CyCMV TP-UL83 and rhCMV Rh112 using immunoassays such as ELISpot and FluoroSpot. The peptide pool induces cytokine secretion from antigen-specific CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The CyCMV+RhCMV (TP-UL83+Rh112) scanning pool induces secretion of e.g., IFN-γ from monkey T cells. It consists of 133 peptides derived from cynomolgus macaque cytomegalovirus (CyCMV) Tegument phosphoprotein UL83 (Uniprot ID: G8H1B8) and 133 peptides derived from rhesus macaque cytomegalovirus (rhCMV) Rh112 (Uniprot ID: Q772N2). The peptides are 15-mers overlapping with 11 amino acids and cover the complete proteins. The mean purity of the peptides is 83%.

The peptide pool has been validated in IFN-γ ELISpot and FluoroSpot using PBMC from cynomolgus and rhesus macaques.

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Product details

Product	PepPool: CyCMV+RhCMV (TP-UL83+Rh112), scanning
Application	T cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Reactivity	Non-human primates
Supplied in	Lyophilized
Contents	Peptide pool with 133 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at -20 °C or below upon receipt.

Performance

PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.