PepPool: SARS‑CoV‑2 Omicron BA.1 (S1), scanning

PepPool: SARS‑CoV‑2 Omicron BA.1 (S1), scanning

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Peptide pool with 168 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against the spike protein of SARS-CoV-2 (variant Omicron B.1.1.529, linage BA.1) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The SARS-CoV-2 Omicron BA.1 (S1) scanning pool induces secretion of e.g., IFN-γ and IL-2 from human T cells. It contains 168 peptides from the human SARS-CoV-2 virus variant Omicron (B.1.1.529), lineage BA.1. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein. The peptide pool contains mutations: A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, and P681H of Omicron BA.1 spike S1.

The peptide pool has been validated using human PBMC from COVID-19 convalescent individuals and vaccinated individuals.

The pool is supplied in two vials: Pool 1: SARS-CoV-2 (Omicron BA.1) S1 peptides 1-84 (84 peptides) and pool 2: SARS-CoV-2 (Omicron BA.1) S1 peptides 85-168 (84 peptides). The mean purity of the synthetic peptides is 80%.

Peptides included in the peptide pool are listed in the datasheet.

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Product details

ProductPepPool: SARS-CoV-2 Omicron BA.1 (S1), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 168 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

Human PBMC (250,000 cells/well) from a SARS-CoV-2 convalescent individual were incubated for 48 hours in the absence or presence of SARS-CoV-2 (Omicron BA.1) S1 defined peptide (vials 1+2) and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

Datasheet/Protocol
SDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research, Published May 17, 2022

Third vaccine dose important for T cell response against Omicron
Three doses mRNA vaccine had better T cell responses than recipients of two doses. Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B were used to assess T cell immunity in mRNA vaccine recipients.

Featured product, Published April 7, 2022

SARS-CoV-2 Omicron BA.1 peptides
We've released PepPool: SARS-CoV-2 Omicron BA.1 (S1), scanning.

Highlighted research, Published December 16, 2021

SARS-CoV-2-specific immune responses in patients with autoimmune diseases
Patients with rheumatic diseases showed T cell responses comparable to healthy control. Patients with MS and after mRNA vaccination show different memory B cell IgG responses. SARS-CoV-2 specific immune responses in autoimmunity was analyzed using FluoroSpot, ELISpot Path, and Mabtech IRIS.

Highlighted research, Published December 2, 2021

Importance of studying SARS-CoV-2-specific memory B cells
When studying antibodies in circulation, remember that memory B cells might hide out of sight. In this study, detection of SARS-CoV-2-specific T and memory B cells 6 and 15 months after infection.

Highlighted research, Published November 24, 2021

Polyfunctional T cells with Mabtech IRIS
Study of development and persistence of virus-specific cellular and humoral immune responses, during the acute phase and two follow-ups after COVID-19 disease in hospitalized patients. Mabtech’s FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α were analyzed with Mabtech IRIS.

Highlighted research, Published June 30, 2020

T cell immunity in COVID-19
Much emphasis has been put on antibody responses, but also T cells are important in the context of COVID-19. In this study, the authors characterized SARS-CoV-2-specific T cell responses by using an extensive FACS panel, peptide stimulations, tetramer staining, and Mabtech IFN-γ ELISpot.

Highlighted research, Published May 30, 2020

B cell FluoroSpot for sensitive ASC detection
Mabtech IRIS and IgG/IgA/IgM FluoroSpot were used to study antibody-secreting plasma cells (ASCs) specific for the nucleocapsid (N) protein. FluoroSpot was shown to be more sensitive than e.g., ELISA.

Highlighted research, Published May 11, 2020

Longevity of immunity against SARS-CoV-2
Investigation of specific T cell responses to SARS-CoV 11 years after outbreak using Mabtech human IFN-γ ELISpot.
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