PepPool: Vaccinia (CD4), human

3635-1

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Content

Peptide pool with 56 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

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Delivery 4-9 business days

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against vaccinia using the ELISpot and FluoroSpot assays. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The Vaccinia (CD4) peptide pool induces secretion of e.g., IFN-γ from human CD4 T cells. It contains 56 defined peptides from 34 vaccinia virus proteins. The peptides are MHC-II restricted. Of the included peptides, 91% share >90% homology with monkeypox virus. The mean purity of the synthetic peptides is 73%.

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Product details

Product	PepPool: Vaccinia (CD4), human
Application	T cell stimulation, ELISpot, FluoroSpot
Reactivity	Human
Supplied in	Lyophilized
Contents	Peptide pool with 56 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at -20 °C or below upon receipt.

Performance

Human PBMC (500,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: Vaccinia (CD4), human (product code 3635-1) and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS.

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Datasheet/Protocol
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