Rabbit anti-mouse IgM, biotin

Rabbit anti‑mouse IgM, biotin

3845-6-250

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Content

Rabbit anti-mouse IgM, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This antibody enables specific detection of mouse IgM in enzyme immunoassays such as indirect ELISA.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

ProductRabbit anti-mouse IgM, biotin
ApplicationELISA
AnalyteIgM
AntibodyRabbit anti-mouse IgM
ConjugateBiotin
ClonalityPolyclonal
Immunogen

Native mouse IgM

HostRabbit
ReactivityMouse
Specificity

Mouse IgM H-chains

Cross-reactivity

No cross-reactivity with mouse IgG, Ig light chains or non-immunoglobulin serum proteins. May cross-react with immunoglobulins from other species.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Rabbit anti-mouse IgM, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
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IgM

Analyte description

Immunoglobulin M (IgM) is the first antibody isotype (also called class) secreted by the adaptive immune system in response to a foreign antigen. Plasma B cells secrete IgM mainly as pentamers, i.e., five IgM molecules joined together by the J chain. Because IgM is formed early in the immune response and is later replaced by IgG, specific antibodies of the IgM class are characteristic of a recent, ongoing, or chronic infection.  Therefore, IgM represents the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind surface-bound antigens with very high avidity. Immunoglobulin M constitutes approximately 10% of the total immunoglobulin concentration in serum. Resting mature yet naive, B lymphocytes express monomeric IgM as a transmembrane antigen receptor that functions as part of the B cell receptor (BCR).

Alternative namesImmunoglobulin M, IgM, AI326478, Igh-6, Igh-M, Igh6, Igm, muH
Cell typeB cell
Gene ID16019