FluoroSpot

FluoroSpot is the multiplex version of ELISpot. Just like in the ELISpot assay, target proteins secreted by cells are captured by specific antibodies immediately after secretion and throughout the stimulation process, making it an extremely sensitive sandwich assay. The difference to ELISpot lies in the mode of detection: where ELISpot relies on an enzymatic reaction, FluoroSpot utilizes fluorescence. This opens up for multiplex analyses of several analytes simultaneously, enabling studies of cell populations with different functional profiles.

Tutorial, Published September 7, 2023

Step-by-step guide to FluoroSpot
An in-depth guide to the FluoroSpot protocol, including best practices and tricks we've learned over the years.

Behind the scenes, Published July 13, 2023

Webinar recording: Master your next FluoroSpot
Watch our recorded webinar on mastering the FluoroSpot assay.

Featured product, Published November 1, 2023

Our FluoroSpot products
Discover the story of every cell with FluoroSpot, the multiplexed version of ELISpot that captures relevant secretion and combines analytes seamlessly. From basic research to clinical trials, FluoroSpot offers robust scalability for comprehensive studies.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Highlighted research, Published January 24, 2024

A comparison of ELISpot and FluoroSpot with flow cytometry methods
Learn about the advantages of ELISpot and FluoroSpot in assessing cellular immune responses in gene therapy evaluation.

Highlighted research, Published April 5, 2019

FluoroSpot more sensitive than flow cytometry
FluoroSpot assay detected cytokine-secreting cells even when as few as 10 transfected cells were added to the cell suspension. In contrast, at least 5 000 transfected cells were required to detect the cytokines by flow cytometry.

Technote, Published November 23, 2022

Technote: Measuring amount of secreted analyte (RSV)
Combining spot numbers with RSV values enables precise characterization of antigen-induced T cell responses.

Tutorial, Published October 13, 2023

7 common errors in FluoroSpot and how to avoid them
Through our years of FluoroSpot development and supporting researchers like you, we've encountered numerous avoidable errors that can occur. In this guide, we've compiled the most common issues and preventive measures.