ELISA Pro: Mouse apoE
ELISA Pro: Mouse apoE
Components
| Plate | Pre-coated ELISA strip plate (mAb mE1) |
| Detection mAb | mE29, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Native mouse apoE ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Buffers/Solutions | Apo ELISA buffer concentate |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution | |
| Also provided | Adhesive plate covers |
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Performance
| Standard range | 1.56-100 ng/ml |
| Sensitivity | 1 ng/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells. |
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Analyte information
apoE
| Analyte description | Apolipoprotein E (apoE) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism, and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL, and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in the recycling of apoptotic remnants and amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In humans, apoE exists in three isoforms, apoE2, apoE3, and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. A normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml. |
| Alternative names | Apolipoprotein E, apoE, apolipoproteinE, apo E, AI255918, Apo-E |
| Cell type | T cell, Treg, Tfh |
| Gene ID | 11816 |