Anti-human IgE mAbs (107/182/101), biotin

Anti‑human IgE mAbs (107/182/101), biotin

3810-8-250

Publications: 6

Documents

Questions?

Validated for our assays.

Content

Monoclonal antibodies 107/182/101, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 1

Anti-human IgE mAb (107), unconjugated

Product specifications

Intended use

These monoclonal enable specific detection of human IgE in ELISpot.

Serum/Plasma samples

Recommendation

107/182/101 are recommended as detection mAbs in ELISpot in combination with capture mAb 107 (product code 3810-3).

Product details

Product	Anti-human IgE mAbs (107/182/101), biotin
Application	ELISpot
Analyte	IgE
Antibody	107/182/101
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgE
Host	Mouse
Isotype	IgG1/IgG2b/IgG2a
Reactivity	Human
Specificity	Human IgE
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	1 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibodies 107/182/101, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Human IgE mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

Application guide: ELISA

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

Application guide: ELISpot

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (6)

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Analyte information

IgE

Analyte description	The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.
Alternative names	Immunoglobulin E, IgE
Cell type	B cell
Gene ID	3497