ELISpot Flex: Human IgE (ALP)

ELISpot Flex: Human IgE (ALP)

3810-2A

Publications: 6
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb107
Detection mAb107/182/101, biotin
Enzyme conjugateStreptavidin-ALP
StimuliAnti-CD40 mAb (S2C6)
Recombinant human IL-4

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Delivery 4-9 business days

Shipping $0

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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IgE using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

IgE secretion can be induced by pre-activation of cells with the included anti-CD40 mAb and IL-4. Please note that stimulation with anti-CD40/IL-4 induces switching to IgE and IgG1 and results with stimulated cells may therefore not be representative of the situation in vivo.

Recommendation

Product details

ProductELISpot Flex: Human IgE (ALP)
ApplicationELISpot
AnalyteIgE
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store capture mAb, detection mAb, and enzyme conjugate at 4-8 °C upon receipt. Anti-CD40 mAb and IL-4 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
PBMC were pre-incubated for 5 days in the absence or presence of anti-CD40 monoclonal antibody and recombinant IL-4. The cells were then washed, added into ELISpot wells and analyzed for the secretion of total IgE (250,000 cells/well, overnight incubation).
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

 

Application guide: ELISA

 

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

IgE Ag

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

IgE total

 

Application guide: ELISpot

 

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

ELISpot IgE

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE
Cell typeB cell
Gene ID3497