Anti-human IgG3 mAb (MTG34), unconjugated

Anti‑human IgG3 mAb (MTG34), unconjugated

3853-3-250

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Content

Monoclonal antibody MTG34, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IgG3 mAb (MTG34), biotin


Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgG3 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MTG34 is recommended as capture mAb in ELISpot (detection with biotinylated antigen). The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-human IgG3 mAb (MTG34), unconjugated
ApplicationELISpot, ELISA
AnalyteIgG3
AntibodyMTG34
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative human IgG3
HostMouse
IsotypeIgG1
ReactivityHuman
SpecificityHuman IgG3
Cross-reactivityNo cross-reactivity (less than 0.1%) with human IgG1, IgG2, IgG4, IgA, IgE, IgM or with IgG from mouse, rat, goat, rabbit and cow. Cross-reactivity with IgG from rhesus and cynomolgus macaque is less than 1%.
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MTG34, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG3

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG3 binds to Fc receptors with high affinity and is a good complement activator.

Alternative namesImmunoglobulin G3, IgG3
Cell typeB cell
Gene ID3502