Anti-human IgG3 mAb (MTG34), biotin

3853-6-250

Publications: 1

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Validated for our assays.

Content

Monoclonal antibody MTG34, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IgG3 mAb (MTG34), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgG3 in ELISpot and antigen-specific ELISA.

Serum/Plasma samples

Recommendation

MTG34 is recommended as detection mAb in ELISpot in combination with capture mAb MT145 (product code 3850-1) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-human IgG3 mAb (MTG34), biotin
Application	ELISpot, ELISA
Analyte	IgG3
Antibody	MTG34
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgG3
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Human IgG3
Cross-reactivity	No cross-reactivity (less than 0.1%) with human IgG1, IgG2, IgG4, IgA, IgE, IgM or with IgG from mouse, rat, goat, rabbit and cow. Cross-reactivity with IgG from rhesus and cynomolgus macaque is less than 1%.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MTG34, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (1)

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Analyte information

IgG3

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG3 binds to Fc receptors with high affinity and is a good complement activator.
Alternative names	Immunoglobulin G3, IgG3
Cell type	B cell
Gene ID	3502