Anti-human IgG4 mAb (MTG42), biotin

Anti‑human IgG4 mAb (MTG42), biotin

3854-6-250

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Content

Monoclonal antibody MTG42, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

Shipping $0

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgG4 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MTG42 is recommended as detection mAb in ELISpot in combination with capture mAb MT145 (product code 3850-1) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

ProductAnti-human IgG4 mAb (MTG42), biotin
ApplicationELISpot, ELISA
AnalyteIgG4
AntibodyMTG42
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative human IgG
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Human IgG4

Cross-reactivityNo cross-reactivity (less than 0.1%) with human IgG1, IgG2, IgG3, IgA, IgE, IgM or with IgG from mouse, rat, goat, and rabbit. Low cross-reactivity with bovine IgG (1.5%) and IgG from rhesus and cynomolgus macaque (<1%).
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTG42, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgG4. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgG4 antibody-secreting cells.

 

Application guide: ELISpot

 

Ag-specific IgG4

Coating:
Antigen
Detection mAb:
MTG42, biotin

human igg4

 

Coating:
MTG42, unconjugated
Detection mAb:
Antigen, biotin

human igg4 2

Total IgG4

Coating:
MT145, unconjugated
Detection mAb:
MTG42, biotin

human igg4 3

 

 

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG4

Analyte description

IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. IgG4 cannot activate the complement system but binds well to Fc receptors.

Alternative namesImmunoglobulin G4, IgG4
Cell typeB cell
Gene ID3503
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