Anti-human IgG4 mAb (MTG42), biotin

3854-6-250

Publications: 2

Documents

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Validated for our assays.

Content

Monoclonal antibody MTG42, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Complementary products

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Anti-human IgG mAb (MT145), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgG4 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MTG42 is recommended as detection mAb in ELISpot in combination with capture mAb MT145 (product code 3850-1) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-human IgG4 mAb (MTG42), biotin
Application	ELISpot, ELISA
Analyte	IgG4
Antibody	MTG42
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgG
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Human IgG4
Cross-reactivity	No cross-reactivity (less than 0.1%) with human IgG1, IgG2, IgG3, IgA, IgE, IgM or with IgG from mouse, rat, goat, and rabbit. Low cross-reactivity with bovine IgG (1.5%) and IgG from rhesus and cynomolgus macaque (<1%).
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MTG42, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Human IgG4 mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgG4. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgG4 antibody-secreting cells.

Application guide: ELISpot

Ag-specific IgG4

Coating:
Antigen
Detection mAb:
MTG42, biotin

Coating:
MTG42, unconjugated
Detection mAb:
Antigen, biotin

Total IgG4

Coating:
MT145, unconjugated
Detection mAb:
MTG42, biotin

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (2)

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Analyte information

IgG4

Analyte description	IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. IgG4 cannot activate the complement system but binds well to Fc receptors.
Alternative names	Immunoglobulin G4, IgG4
Cell type	B cell
Gene ID	3503