Anti-human TNF-α mAb (MT15B15), biotin

Anti‑human TNF‑α mAb (MT15B15), biotin

3512-2-250

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Content

Monoclonal antibody MT15B15, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human TNF-α in flow cytometry and Western blot.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

ProductAnti-human TNF-α mAb (MT15B15), biotin
ApplicationFlow cytometry, Western blot
AnalyteTNF-α
AntibodyMT15B15
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human TNF-α
HostMouse
IsotypeIgG1
ReactivityHuman
SpecificityNative and recombinant human TNF-α
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT15B15, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Detection of human TNF-α by Western blot. E.coli derived human TNF-α (10 ng and 45 ng) detected by 1 ug/ml MT15B15, biotinylated followed by SA-HRP and TMB substrate.
Datasheet/Protocol
SDS
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124
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Anti-human TNF-α mAb (MT15B15), PF488P