Anti-human TNF-α mAb (MT15B15), PF488P

3512-71-100T

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Isotype control mAb (MT128) for mouse IgG1, PF488P

Product specifications

Intended use

This monoclonal antibody enables specific detection of human TNF-α-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.

Product details

Product	Anti-human TNF-α mAb (MT15B15), PF488P
Application	Flow cytometry
Analyte	TNF-α
Antibody	MT15B15
Conjugate	PF488P
Clonality	Monoclonal
Immunogen	Recombinant human TNF-α
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human TNF-α
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT15B15, conjugated to PF488P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of TNF-α by flow cytometry in viable human lymphocytes. Stimulated, fixed and permeabilized cells were stained with anti-human TNF-α mAb MT15B15-PF488P in combination with anti-CD3-PE (left panel). Matched isotype control antibody (right panel). Flow cytometry was performed on a BD FACSVerse system.

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Analyte information

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124