Anti-influenza B (NP) mAb (4D5), biotin

Anti-influenza B (NP) mAb (4D5), biotin

4406-6-100

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Product specifications

Intended use

This monoclonal antibody enables specific detection of the influenza B nucleoprotein (NP) in FociSpot immunostaining of virus infected cells. The antibody can also be used in ELISA.

Serum/Plasma samples

Applications
FociSpot immunostaining of influenza B nucleoprotein in focus forming assays. Antibody functionality in FociSpot was confirmed using influenza B infected MDCK cells.

Functionality in ELISA was confirmed using recombinant protein.

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Product details

ProductAnti-influenza B (NP) mAb (4D5), biotin
ApplicationFociSpot, ELISA
AnalyteInfluenza B (NP)
Antibody4D5
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant influenza B nucleoprotein

HostMouse
IsotypeIgG1
Specificity

Native and recombinant influenza B nucleoprotein

Purification

Purified by protein A affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody 4D5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of influenza B nucleoprotein in infected MDCK cells. MDCK cells were infected with influenza B virus (titrated, virus dilutions16,000x, 32,000x, and 64,000x are shown). After fixation and permeabilization, foci were detected using anti-influenza B (NP) mAb 4D5, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/ProtocolSDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Featured product, Published June 21, 2023

Influenza B FociSpot kit is here
The nucleoprotein of Influenza B can be detected with our new monoclonal antibody, which is included in our corresponding FociSpot kit.
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Influenza B (NP)

Analyte description

The Nucleoprotein (NP) is a structural protein and encapsidates the negative strand RNA of influenza B into ribonucleoproteins (RNP). NP plays a role in replication, transcription, RNA packaging, and nuclear trafficking. The NP does not only interact with the RNA, but also the viral polymerase, the M protein, and several host factors. 

Alternative namesNucleoprotein, NP (of influenza B)