Anti-mouse Ig κ chain mAb (MT24), biotin

Anti‑mouse Ig κ chain mAb (MT24), biotin

3875-6-250

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Content

Monoclonal antibody MT24, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse Ig κ chain mAb (MT24), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse Ig kappa chains in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT24 is recommended as detection mAb in ELISA and ELISpot with coated antigen.

Product details

ProductAnti-mouse Ig κ chain mAb (MT24), biotin
ApplicationELISpot, ELISA
AnalyteIg
AntibodyMT24
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative mouse Ig
HostRat
IsotypeIgG1
ReactivityMouse
SpecificityMouse Ig kappa light chain. Kappa chains generally represent more than 90% of mouse Ig light chains.
Cross-reactivityMT24 does not detect the lambda (λ) light chain. No cross-reactivity (less than 0.1%) with Ig from rat, goat, rabbit, cow, sheep, horse, or human.
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT24, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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Ig

Analyte description

An antibody (also called immunoglobulin) is a glycoprotein expressed by B cells. Each antibody consists of four polypeptide chains, with two identical copies of both the light and heavy chains. Intra- and inter-chain disulfide bonds link the four polypeptide chains together to one antibody. The heavy chain decides the antibody’s isotype and subclass. The light chains of an antibody are identical and can be of two types; kappa (κ) or lambda (λ). The ratio of kappa: lambda in serum immunoglobulins varies significantly among species. 

Alternative namesImmunoglobulin, Ig, kappa
Cell typeB cell
Gene ID243469